| Anne Blake |
Burgomaster's Wife |
| Henry Caine |
Schoolmaster |
| Hazel Court |
Elizabeth |
| Peter Cushing |
Baron Victor Frankenstein |
| Hugh Dempster |
Burgomaster |
| Valerie Gaunt |
Justine |
| Paul Hardtmuth |
Prof. Bernstein |
| Melvyn Hayes |
Young Victor |
| Noel Hood |
Aunt Sophia |
| Marjorie Hume |
Mother |
| Ernest Jay |
Undertaker |
| Claude Kingston |
Little Boy |
| Eugene Leahy |
Second Priest |
| Christopher Lee |
The Creature |
| Michael Mulcaster |
Warder |
| Bartlett Mullins |
Tramp |
| Raymond Rollett |
Father Felix |
| Patrick Troughton |
Kurt |
| Robert Urquhart |
Paul Krempe |
| Sally Walsh |
Elizabeth (as child) |
| Fred Johnson |
Grandfather |
| Joseph Behrman |
Fritz |
| Alex Gallier |
Priest |
| Sally Walsh |
Elizabeth (as child) |